A semi-defined growth medium for Renibacterium salmoninarum
نویسندگان
چکیده
منابع مشابه
Charcoal agar, a new growth medium for the fish disease bacterium Renibacterium salmoninarum.
Charcoal is an effective replacement for serum in media for the isolation and culture of Renibacterium salmoninarum, the causative agent of bacterial kidney disease in salmonid fish. The medium, KDM-C, contains 10 g of peptone, 0.5 g of yeast extract, 1 g of L-cysteine hydrochloride, 1 g of activated charcoal, and 15 g of agar per liter and is adjusted to pH 6.8 with NaOH before autoclaving. Ei...
متن کاملFurther characterization of Renibacterium salmoninarum extracellular products.
Renibacterium salmoninarum, the agent of bacterial kidney disease in salmonids, releases high concentrations of extracellular protein in tissues of infected fish. The extracellular protein consists almost entirely of a 57-kDa protein and derivatives of degradation and aggregation of the same molecule. The 57-kDa protein and its derivatives were fractionated into defined ranges of molecular mass...
متن کاملFlavobacterium psychrophilum, Renibacterium salmoninarum and Aeromonas salmonicida
Intra-ovum infection of artificialy contaminated salmonid (rainbow trout or amago salmon) eggs was studied using three fish pathogens (Flavobacterium psychrophilum, Renibacterium salmoninarum and Aeromonas salmonicida). Artificial contamination was performed using egg-surface contamination or immersion-water contamination. For F. psychrophilum and R. salmoninarum, infection occurred by the entr...
متن کاملPerformance of serum-free broth media for growth of Renibacterium salmoninarum.
Growth of Renibacterium salmoninarum was compared in 14 different broth media; 13 serum-free, and 1 that contained newborn calf serum, KDM2+M. Supplementation with 1% v/v R. salmoninarum MCO4M metabolite was evaluated for 6 of the media that do not utilize it as part of their ingredients. Viable cells were enumerated on Days 10, 20, and 30 post inoculation to evaluate performance. The experimen...
متن کاملGenetic Variation of Renibacterium salmoninarum Genes in Infected Salmonids
I understand that my project will become part of the permanent collection of Oregon State University, Bioresource Research. My signature below authorizes release of my project to any reader upon request. Abstract Renibacterium salmoninarum is the causative agent of bacterial kidney disease in both wild and farmed salmonid species worldwide. The genome of this pathogen has significant synteny to...
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ژورنال
عنوان ژورنال: FEMS Microbiology Letters
سال: 1982
ISSN: 0378-1097
DOI: 10.1016/0378-1097(82)90225-7